home
***
CD-ROM
|
disk
|
FTP
|
other
***
search
/
The Arsenal Files 6
/
The Arsenal Files 6 (Arsenal Computer).ISO
/
health
/
med9603.zip
/
M9630541.TXT
< prev
next >
Wrap
Text File
|
1996-02-27
|
1KB
|
26 lines
Document 0541
DOCN M9630541
TI Prior enrichment of human immunodeficiency virus DNA with probe DNA
particles for efficient PCR diagnosis.
DT 9603
AU Tano H; Fan K; Kitajima MA; Kasai K; Tamatsukuri S; Furuichi Y; Hayashi
T; Kondo M; Imai M; Tsukuba Research Laboratory, Japan Synthetic Rubber
Co., Ltd.,; Japan.
SO J Clin Microbiol. 1995 Sep;33(9):2489-91. Unique Identifier : AIDSLINE
MED/96025179
AB We report here a novel method by which one can recover human
immunodeficiency virus (HIV) DNA in a small volume (approximately 50
microliters) of solution from 8 ml of crude cell lysate which contains
as few as several HIV DNA copies. The method uses the specific binding
of HIV DNA to HIV probe DNA particles. The HIV DNA thus concentrated on
the particles can be subjected to the PCR assay. The method also enables
the examination of 100 individual blood specimens in a combined form.
DE Base Sequence DNA, Viral/*ANALYSIS Human HIV/GENETICS/*ISOLATION &
PURIF HIV Seropositivity/DIAGNOSIS Molecular Probe Techniques
Molecular Sequence Data Polymerase Chain Reaction/*METHODS JOURNAL
ARTICLE
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).